Interesting things

Some useful papers and links about the ITS RFLPs for identifying yeasts and about yeast phylogeny can be found in the following references and sections.

    • Belloch C., Barrio E., García M.D. and Querol A. (1998). Phylogenetic reconstruction of the yeast genus Kluyveromyces: restriction map analysis of the 5.8S rRNA gene and the two ribosomal internal transcribed spacers. Syst Appl Microbiol., 21(2):266-73.
    • de Llanos Frutos R., Fernández-Espinar M.T. and Querol A. (2004). Identification of species of the genus Candida by analysis of the 5.8S rRNA gene and the two ribosomal internal transcribed spacers. Antonie van Leeuwenhoek 85:175–185.
    • Esteve-Zarzoso B., Belloch C., Uruburu F. and Querol A. (1999). Identification of yeasts by RFLP analysis of the 5.8S rRNA gene and the two ribosomal internal transcribed spacers. Int. J. Syst. Bacteriol., 49 (Pt 1):329–337.
    • Esteve-Zarzoso B., Peris-Torán M.J., Ramón D. and Querol A. (2001). Molecular characterisation of Hanseniaspora Species. Antonie van Leeuwenhoek 80:85–92.
    • Fernández-Espinar M.T., Esteve-Zarzoso B., Querol A. and Barrio E. (2000). RFLP analysis of the ribosomal internal transcribed spacers and the 5.8S rRNA gene region of the genus Saccharomyces: a fast method for species identification and the differentiation of flor yeasts. Antonie van Leeuwenhoek, 78:87–97.
    • Guillamón JM., Sabaté J., Barrio E., Cano J. & Querol A. (1998). Rapid identification of wine yeasts species based on RFLP analysis of the ribosomal ITS regions. Arch. Microbiol., 169:387–392.
    • Villa-Carvajal M. Querol A. and Belloch C. (2006). Identification of species in the genus Pichia by restriction of the internal transcribed spacers (ITS1 and ITS2) and the 5.8S ribosomal DNA gene. Antonie van Leeuwenhoek 90:171–181.
    • Casaregola S., Weiss S., Morel G. (2011). New perspectives in hemiascomycetous yeast taxonomy. C.R. Biologies 334:590–598.
    • Kurtzman C.P. and Robnett C.J. (1997). Identification of clinically important ascomycetous yeasts based on nucleotide divergence in the 5' end of the large-subunit (26S) ribosomal DNA gene. J. Clin. Microbiol., 35:1216–1223.
    • Kurtzman C.P. and Robnett C.J. (1998). Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 3:331–371.
    • Kurtzman C.P., Robnett C.J. (2003). Phylogenetic relationships among yeasts of the ‘Saccharomyces complex’ determined from multigene sequence analyses. FEMS Yeast Res., 3: 417–432.
    • Kurtzman C.P. (2003). Phylogenetic circumscription of Saccharomyces, Kluyveromyces and other members of the Saccharomycetaceae, and the proposal of the new genera Lachancea, Nakaseomyces, Naumovia, Vanderwaltozyma and Zygotorulaspora. FEMS Yeast Res., 4:233–245.
    • Kurtzman C.P., Robnett C.J., Ward J.M., Brayton C., Gorelick P., Walsh T.J. (2005). Multigene phylogenetic analysis of pathogenic candida species in the Kazachstania (Arxiozyma) telluris complex and description of their ascosporic states as Kazachstania bovina sp. nov., K. heterogenica sp. nov., K. pintolopesii sp. nov., and K. slooffiae sp. nov. J. Clin. Microbiol. 43:101–111.
    • Kurtzman C.P. (2006). New species and new combinations in the yeast genera Kregervanrija gen. nov., Saturnispora and Candida, FEMS Yeast Res., 6:288–297.
    • Kurtzman C.P., Albertyn J., Basehoar-Powers E. (2007). Multigene phylogenetic analysis of the Lipomycetaceae and the proposed transfer of Zygozyma species to Lipomyces and Babjevia anomala to Dipodascopsis, FEMS Yeast Res., 7:1027–1034.
    • Kurtzman C.P., Robnett C.J. (2007). Multigene phylogenetic analysis of the Trichomonascus, Wickerhamiella and Zygoascus yeast clades, and the proposal of Sugiyamaella gen. nov. and 14 new species combinations. FEMS Yeast Res., 7:141–151.
    • Kurtzman C.P., Robnett C.J., Basehoar-Powers E. (2008). Phylogenetic relationships among species of Pichia, Issatchenkia and Williopsis determined from multigene sequence analysis, and the proposal of Barnettozyma gen. nov., Lindnera gen. nov. and Wickerhamomyces gen. nov. FEMS Yeast Res. 8:939–954.
    • Kurtzman C.P., Robnett C.J. (2010). Systematics of methanol assimilating yeasts and neighboring taxa from multigene sequence analysis and the proposal of Peterozyma gen. nov., a new member of the Saccharomycetales. FEMS Yeast Res., 10:353–361.
    • Kurtzman C.P. (2011). Phylogeny of the ascomycetous yeasts and the renaming of Pichia anomala to Wickerhamomyces anomalus. Antonie Van Leeuwenhoek 99:13–23
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YeastIP is a comprehensive gene database which contains the major barcodes of all the species of the subphylum Saccharomycotina and tools to establish the identification of yeasts to the species level and their phylogenetic relationships.


MycoBank database

MycoBank is owned by the International Mycological Association and is an on-line database aimed as a service to the mycological and scientific community by documenting mycological nomenclatural novelties (new names and combinations) and associated data, for example descriptions and illustrations. Pairwise sequence alignments and polyphasic identifications of fungi and yeasts against curated references databases are proposed.