A quick identification method based on restriction analysis of the ITS region



In the late nineteen nineties the development of a new method by the CECT-IATA Associated unit to identify yeasts, based on restriction analysis of the region that includes the gene codifying 5,8S rRNA and the transcribed intergenic regions ITS (5,8S-ITS), enabled fast identification of strains belonging to a great many yeast species (Esteve-Zarzoso et. al., 1999). The data generated through experiments carried out using this technique led to the development of a database for rapid identification of yeasts. This data base is expanding continually and by consulting it one can identify more than 200 species both quickly and simply.

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